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商品详细Biothema/ATP试剂SL/玻璃瓶中的冻干试剂,在氩气/11-501下密封
Biothema/ATP试剂SL/玻璃瓶中的冻干试剂,在氩气/11-501下密封
Biothema/ATP试剂SL/玻璃瓶中的冻干试剂,在氩气/11-501下密封
商品编号: 11-501
品牌: Biothema
市场价: ¥12240.00
美元价: 6120.00
产地: 美国(厂家直采)
公司:
产品分类: 逆转录试剂盒
公司分类: Sensiscript_RT_Kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description

Applications

ATP Reagent SL is a Stable Light (SL) reagent with a decay of ATP and light emission of less than 1% per minute. The stable light allows manual handling and you can use a simpler luminometer. The detection limit is 10-15 mole ATP, making the reagent suitable for assays of mammalian cells and for the continous monitoring of enzymatic reactions.

Product characteristics

Delivery form: Lyophilised reagent in a glass vial, sealed under argon

Reconstitution: In 23-101 Diluent C. A further 5 fold dilution in Tris-EDTA buffer or other suitable buffer is recomended, for maximum performance.

Number of assays: Up to 250 analyses in a 96 well microplate format or 50 analyses in cuvette format.

Linear range: From 10-15 to 10-12 mole ATP, depending on the linearity of the luminometer.

Storage: +4°C

Shelf life: 2 years from date of delivery at +4°C. Reconstituted reagent should be used within a week.

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品牌介绍
BioThema完全专注于萤火虫荧光素酶反应的分析应用,无论是检测细胞,测量酶活性还是其他。在该反应中,萤光素被ATP(三磷酸腺苷)活化,然后被氧化成氧萤光素和CO 2。氧化荧光素以激发态形成,并在进入基态时发光。通常在所谓的发光计中测量光。ATP +萤光素+ O 2-萤光素酶-> AMP +焦磷酸盐+氧化萤光素+ CO 2 +光在ATP的测定中,光发射可以基本恒定或迅速衰减。因此,存在基于萤火虫荧光素酶反应的稳定的光和闪光型ATP试剂。在萤光素酶含量低的情况下,每分钟消耗的ATP比例可忽略不计,因此光几乎可以保持恒定,但也很低。在荧光素酶水平高的情况下,不可避免地会消耗ATP,导致光线迅速减少,但最初的峰值光线很高。实际上,峰高和衰减率均与萤光素酶活性成正比