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商品详细Biothema/ATP消除试剂/单试剂/71-101
Biothema/ATP消除试剂/单试剂/71-101
Biothema/ATP消除试剂/单试剂/71-101
商品编号: 71-101
品牌: Biothema
市场价: ¥8520.00
美元价: 4260.00
产地: 美国(厂家直采)
公司:
产品分类: 簇生成和测序试剂
公司分类: sequencing_reagents
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description

Applications

ATP Eliminating Reagent is used to quickly degrade extracellular ATP in a sample. The ATP concentration is reduced with 50% per second. For biological samples, please allow up to 10 minutes for complete degradation of extracellular ATP. ATP Eliminating Reagent should be reconstituted in either 23-101 Diluent C or in 28-101 Cell Lysing Reagent, if mammalian cells should be eliminated at the same time.

Product characteristics

Delivery form: Single reagent

Number of assays: Depending on assay and sample. For further guidence please contact us at info@biothema.com.

Delivery form: Lyophilised reagent in a glass vial, sealed under argon

Storage: +4°C

Shelf life: 5 years from date of delivery at +4°C.

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品牌介绍
BioThema完全专注于萤火虫荧光素酶反应的分析应用,无论是检测细胞,测量酶活性还是其他。在该反应中,萤光素被ATP(三磷酸腺苷)活化,然后被氧化成氧萤光素和CO 2。氧化荧光素以激发态形成,并在进入基态时发光。通常在所谓的发光计中测量光。ATP +萤光素+ O 2-萤光素酶-> AMP +焦磷酸盐+氧化萤光素+ CO 2 +光在ATP的测定中,光发射可以基本恒定或迅速衰减。因此,存在基于萤火虫荧光素酶反应的稳定的光和闪光型ATP试剂。在萤光素酶含量低的情况下,每分钟消耗的ATP比例可忽略不计,因此光几乎可以保持恒定,但也很低。在荧光素酶水平高的情况下,不可避免地会消耗ATP,导致光线迅速减少,但最初的峰值光线很高。实际上,峰高和衰减率均与萤光素酶活性成正比