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商品详细Biothema/ATP试剂SS/玻璃瓶中的冻干试剂,在氩气/13-101下密封
Biothema/ATP试剂SS/玻璃瓶中的冻干试剂,在氩气/13-101下密封
Biothema/ATP试剂SS/玻璃瓶中的冻干试剂,在氩气/13-101下密封
商品编号: 13-101
品牌: Biothema
市场价: ¥36700.00
美元价: 18350.00
产地: 美国(厂家直采)
公司:
产品分类: ELISA辅助试剂
公司分类: ELISA_helper_reagent
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description

Applications

ATP Reagent SS is a Super Sensitive (SS) reagent which under the right conditions (absolutely ATP free cuvettes etc) allows you to detect down to 10-18 mole ATP, the amount of ATP found in a single bacterial cell. http://www.titertek-berthold.com/application-notes/download/atp-detection-limit-biothema-application-note.html. The light emission is very high, however not stable with a decay of 235% per minute. The low stability makes it necessary to use the reagent with a luminometer with dispensers.

Product characteristics

Delivery form: Lyophilised reagent in a glass vial, sealed under argon

Reconstitution: In 22-101 Diluent B or in 24-101 Diluent D depending wheter protection against Extractant B/S is required or not.

Number of assays: Up to 100 analyses in test tube format.

Linear range: From 10-18 to 10-13 mole ATP, depending on the linearity of the luminometer.

Storage: +4°C

Shelf life: 2 years from date of delivery at +4°C. Reconstituted reagent should be used within a week.

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品牌介绍
BioThema完全专注于萤火虫荧光素酶反应的分析应用,无论是检测细胞,测量酶活性还是其他。在该反应中,萤光素被ATP(三磷酸腺苷)活化,然后被氧化成氧萤光素和CO 2。氧化荧光素以激发态形成,并在进入基态时发光。通常在所谓的发光计中测量光。ATP +萤光素+ O 2-萤光素酶-> AMP +焦磷酸盐+氧化萤光素+ CO 2 +光在ATP的测定中,光发射可以基本恒定或迅速衰减。因此,存在基于萤火虫荧光素酶反应的稳定的光和闪光型ATP试剂。在萤光素酶含量低的情况下,每分钟消耗的ATP比例可忽略不计,因此光几乎可以保持恒定,但也很低。在荧光素酶水平高的情况下,不可避免地会消耗ATP,导致光线迅速减少,但最初的峰值光线很高。实际上,峰高和衰减率均与萤光素酶活性成正比